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SRX1626372: unc-54(cc3389) Ribo-seq
1 ILLUMINA (Illumina MiSeq) run: 3.2M spots, 176.1M bases, 75.4Mb downloads

Design: Ribosome footprint profiling Ribo-seq, using 3'ligation with AF-JA-34.2 and circular DNA ligase.
Submitted by: Stanford Univerisy
Study: Translation Readthrough Mitigation
show Abstracthide Abstract
A fraction of ribosomes engaged in translation will fail to terminate when reaching a stop codon, yielding nascent proteins inappropriately extended on their C-termini. Although such extended proteins can interfere with normal cellular processes, known mechanisms of translational surveillance are insufficient to protect cells from potential dominant consequences. Using C. elegans, we demonstrate a consistent ability of cells to block accumulation of C-terminal extended proteins that result from failure to terminate at stop codons. These repressive effects are mediated through decreased protein accumulation without a detectable effect on mRNA levels. 3’UTR-encoded peptides are sufficient to confer the observed effects, suggesting a co- or post-translational mechanism of action. We suggest 3’UTRs may be optimized for sequences that destabilize the attached protein, providing a surveillance mechanism for unwelcome/inadmissible and varied translation errors.
Sample: unc-54(cc3389) homozygotes
SAMN04546343 • SRS1335846 • All experiments • All runs
Library:
Name: SJA131M
Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Spot descriptor:
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Runs: 1 run, 3.2M spots, 176.1M bases, 75.4Mb
Run# of Spots# of BasesSizePublished
SRR32192643,200,939176.1M75.4Mb2016-05-18

ID:
2323108

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